Picture of FLIM Inverted microscope platform
Current status:
AVAILABLE
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1st Responsible:
2nd Responsible:
Group:
26_Ultrafast Bio- and Nanobiophotonics
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Custom-build Fluorescence Lifetime Imaging Microscope (FLIM) inverted microscope (RM21, MCL), equipped with micro- and nanopositioning system from MCL with trvel ranges of 20 x 20 x 20 mm and 200 x 200 x 200 um, respectively. Equipped with several laser excitation sources (fs and ps lasers, Spectra Physics, Hamamatsu, Becker&Hickl) and with fast electronics for Time Correlated Single Photon Counting (TCSPC) (SPC130, Becker & Hickl), suited filter sets for one photon and multiphoton excitation/detection modes. Gas/humidity and temperature control in incubator for live cell experiments, and Anisotropy and Hanbury Brown Twiss detection schemes implemented as well as possibility to couple the output to a Streak detection system (see Streak Detection unit in LIMS plaform). Flexible platform that allows muultiple configurations also in transmission. Control and analysis software self programmed in Labview and Matlab, respectively.

Tool name:
FLIM Inverted microscope platform
Area/room:
17.00. Ultrafast Nano and Biophotonics Laboratory
Manufacturer:
MadCityLabs, with several upgrades
Model:
RM21
Booking type:
Compulsory

Please follow SOP and work in collaboration with the Ultrafast Bio- and Nanophotonics team to identify best possible system configuration and data acquisition scheme.

Instructors

Licensed Users

Nanophotonics and Bioimaging User Facility
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