Picture of NBI - Circular Dichroism Measurement System
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10_Nanophotonics Flagship Facility
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The Jasco J-815 circular dichroism (CD) spectroscopy measures differences in the absorption of left-handed polarized light versus right-handed polarized light that arise due to structural asymmetry. The absence of regular structure results in zero CD intensity, while an ordered structure results in a spectrum which can contain both positive and negative signals.

CD can be used for: 

  • Determination if a protein is folded
  • Characterization of  secondary structure (α-helix, β-sheet)
  • Detection of changes in structure upon mutagenesis
  • Studying conformational stability of proteins:
    • pH stability
    • denaturant stability (urea, guadinium hydrochloride) 
    • temperature
    • buffers
    • addition of stabilizers
  • Detection of Changes in the conformation of a protein upon protein:protein interaction

Characterization of secondary structure is probably the most used CD spectroscopy application. Secondary structure can be identified in the "far-UV" spectral region (190-250 nm). The protein peptide bond is the chromophore, and it is possible to detect a signal if the protein is in a specific secondary structural conformation (α-helix, β-sheet).

Tool name:
NBI - Circular Dichroism Measurement System
12.00. Optical spectroscopy and analysis
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Nanophotonics and Bioimaging User Facility
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