Our in-house built combined fluorescence and AFM microscopg techniques offers the life science research community a powerful tool to investigate complex biological systems and their interactions. This combined microscopy platform is based on fluorescence optical sectioning microscopy through aperture correlation microscopy with a Differential Spinning Disk (DSD), and nanomechanical mapping with an Atomic Force Microscope (AFM). The illumination scheme of the DSD microscope unit, contrary to standard single or multi-point confocal microscopes, provides a time-independent illumination of the AFM cantilever. This enables a distortion-free simultaneous operation of fluorescence optical sectioning microscopy and atomic force microscopy with standard probes.